The argyrophilic nucleolar organizer regions in ductal carcinoma in situ of the breast. The significance of ploidy and proliferative activity analysis using this silver staining technique

Cancer ◽  
1994 ◽  
Vol 74 (6) ◽  
pp. 1739-1745 ◽  
Author(s):  
Walid A. Mourad ◽  
Sebouh Setrakian ◽  
Michele L. Hales ◽  
Mohamed Abdulla ◽  
Giuliana Trucco
Keyword(s):  
Carcinoma In Situ ◽  
Proliferative Activity ◽  
Ductal Carcinoma ◽  
Nucleolar Organizer ◽  
Staining Technique ◽  
Activity Analysis ◽  
Nucleolar Organizer Regions ◽  
Silver Staining Technique ◽  
Argyrophilic Nucleolar Organizer Regions

scholarly journals Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR).

1993 ◽  
Vol 41 (3) ◽  
pp. 439-445 ◽  
Author(s):  
L E Lindner
Keyword(s):  
Silver Staining ◽  
Nucleolar Organizer ◽  
Staining Technique ◽  
Nucleolar Organizer Regions ◽  
Silver Stain ◽  
Oxidation Reduction ◽  
Background Staining ◽  
Silver Staining Technique ◽  
Selection Of ◽  
Nucleolar Function

The silver-staining technique for nucleolar organizer regions (AgNOR) of Ploton et al., as popularized by Crocker, is being widely used for evaluation of nucleolar function, especially in neoplasia. It suffers from limited reliability, background staining, precipitates, and fading of the sections. Factors were identified that affect these problems. The oxidation-reduction level and gelatin used are particularly important. An improved procedure is presented which incorporates pre-reduction of the sections, selection of an optimal gelatin, and post-treatment of the sections to produce a permanent preparation. It is compatible with many fixatives and with other stains used before or after the silver stain.

Studies on argyrophilic nucleolar organizer regions (AgNORs) in a spontaneous mammary gland ductal carcinoma of a captive rhesus monkey

2007 ◽  
Vol 58 (5) ◽  
pp. 361-365
Author(s):  
Beatriz Goldschmidt ◽  
Renato Sergio Marchevsky ◽  
Marcia Cristina Ribeiro Andrade ◽  
Claudia Andréia de Araújo Lopes ◽  
Miguel Ângelo Bruck Gonçalves ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Rhesus Monkey ◽  
Ductal Carcinoma ◽  
Nucleolar Organizer ◽  
Nucleolar Organizer Regions ◽  
Argyrophilic Nucleolar Organizer Regions
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